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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AB0016-500 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0016-500, RRID:AB_2333134
- Product name
- Anti-CX43
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 2 mg
- Concentration
- 4 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin.
Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart.
AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions.
Martins-Marques T, Pinho MJ, Zuzarte M, Oliveira C, Pereira P, Sluijter JP, Gomes C, Girao H
Journal of extracellular vesicles 2016;5:32538
Journal of extracellular vesicles 2016;5:32538
Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart.
Martins-Marques T, Anjo SI, Pereira P, Manadas B, Girão H
Molecular & cellular proteomics : MCP 2015 Nov;14(11):3040-55
Molecular & cellular proteomics : MCP 2015 Nov;14(11):3040-55
AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions.
Ribeiro-Rodrigues TM, Catarino S, Marques C, Ferreira JV, Martins-Marques T, Pereira P, Girão H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4629-41
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4629-41
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cell lysate at 100 µg per lane
- Sample type
- COS-7 cell line
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cells were fixed with methanol
- Sample type
- primary RPE cells
- Primary Ab dilution
- 1/100
- Conjugate
- Unconjugated
