Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-37584 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Connexin 43 (Tyr265) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Connexin 43 antibody detects endogenous levels of Connexin 43 only when phosphorylated at Tyrosine 265. A suggested positive control for Western blot is HUVEC cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Vinpocetine Protects Against Cerebral Ischemia-Reperfusion Injury by Targeting Astrocytic Connexin43 via the PI3K/AKT Signaling Pathway.
Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone.
Zhao M, Hou S, Feng L, Shen P, Nan D, Zhang Y, Wang F, Ma D, Feng J
Frontiers in neuroscience 2020;14:223
Frontiers in neuroscience 2020;14:223
Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone.
Yin X, Feng L, Ma D, Yin P, Wang X, Hou S, Hao Y, Zhang J, Xin M, Feng J
Journal of neuroinflammation 2018 Mar 27;15(1):97
Journal of neuroinflammation 2018 Mar 27;15(1):97
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HUVEC cells using Connexin 43 (pTyr265) polyclonal antibody (Product # PA5-37584) (left) or the same antibody preincubated with a blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 Effects of Vinp on inflammation and apoptosis-related proteins, and the expression of Cx43, p-Cx43, AKT, and p-AKT in the rat cerebral cortex after I/R injury. (A) Representative western blot images and quantification of proteins related to inflammation. (B) Representative western blot images and quantification of proteins related to apoptosis. (C) Representative western blot images of Cx43 and p-Cx43, and quantification of Cx43, p-Cx43, and p-Cx43/Cx43. (D) Representative western blot images of AKT and p-AKT, and quantification of AKT, p-AKT and p-AKT/AKT. Data are shown as the mean +- SEM ( n = 5 in each group). ** P < 0.01, *** P < 0.001 using one-way ANOVA followed by Tukey's post hoc tests. Vinp: Vinpocetine; LY: LY294002; I/R: ischemia/reperfusion.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 Effects of Vinp on the expression of Cx43, p-Cx43, AKT, and p-AKT in astrocytes after OGD/R. (A) Representative western blot images of Cx43 and p-Cx43, and quantification of Cx43, p-Cx43, and p-Cx43/Cx43. (B) Representative western blot images of AKT and p-AKT, and quantification of AKT, p-AKT and p-AKT/AKT. Data are shown as the mean +- SEM ( n = 3 in each group). * P < 0.05, ** P < 0.01, *** P < 0.001 using one-way ANOVA followed by Tukey's post hoc tests. Vinp: Vinpocetine; LY: LY294002; OGD/R: oxygen-glucose deprivation/reoxygenation; BKM: BKM120.