PAB5681

antibody from Abnova Corporation

Targeting: GJA1 CX43, GJAL, ODD, ODDD, ODOD, SDTY3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB5681

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB5681, RRID:AB_1577918

Product name

GJA1 (phospho S367) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of GJA1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Alterations of phosphorylation state of connexin 43 during hypoxia and reoxygenation are associated with cardiac function.

Matsushita S, Kurihara H, Watanabe M, Okada T, Sakai T, Amano A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2006 Mar;54(3):343-53

---

Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.

Bao X, Altenberg GA, Reuss L
American journal of physiology. Cell physiology 2004 Mar;286(3):C647-54

---

Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation.

Solan JL, Fry MD, TenBroek EM, Lampe PD
Journal of cell science 2003 Jun 1;116(Pt 11):2203-11

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of extract from HeLa (Lane 1, 4), K-562 (Lane 2, 5) and 293 (Lane 3, 6) cells, untreated or treated with PMA (1 uM, 30 min). Using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)