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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PPS046 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-Connexin 43/GJA1 (S368) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. This antibody is specific for the 43 kDa Connexin 43/GJA1 in Western blots of rat brain lysates.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Simian, Zebrafish
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
Submitted references Protein kinase Cδ-mediated phosphorylation of Connexin43 gap junction channels causes movement within gap junctions followed by vesicle internalization and protein degradation.
Cone AC, Cavin G, Ambrosi C, Hakozaki H, Wu-Zhang AX, Kunkel MT, Newton AC, Sosinsky GE
The Journal of biological chemistry 2014 Mar 28;289(13):8781-98
The Journal of biological chemistry 2014 Mar 28;289(13):8781-98
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-Connexin 43/GJA1 (S368) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 43 kDa Connexin 43/GJA1 phosphorylated at S368 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 units of lambda phosphatase ( lambda Phosphatase) for 30 minutes before being exposed to the Anti-Phospho-Connexin 43/GJA1 (S368). The immunolabeling of Connexin43 is completely eliminated by treatment with lambda PPase.
