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LearnM4276
antibody from MilliporeSigma / Merck KGaA
Targeting: MYH2
IBM3, MYH2A, MYHas8, MyHC-2A, MyHC-IIa, MYHSA2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- M4276 - Provider product page
- Provider
- MilliporeSigma / Merck KGaA
- Product name
- Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Feline, Guinea Pig, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- MY-32
- Storage
- Store at -20°C
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 223
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
