AMP00010G

antibody from Leading Biology

Targeting: MYH2 IBM3, MYH2A, MYHas8, MyHC-2A, MyHC-IIa, MYHSA2

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

AMP00010G

Provider

Leading Biology

Product name

Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)

Antibody type

Monoclonal

Description

anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Reactivity

Human, Mouse, Rat, Drosophila

Antibody clone number

SF9

Storage

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.