- PA1-777 - Invitrogen Antibodies TPH1 antibody

Battaglia DM, Sanchez-Pino MD, Nichols CD, Foster TP
Frontiers in microbiology 2022;13:859866

Chen Y, Xu H, Zhu M, Liu K, Lin B, Luo R, Chen C, Li M
Oncotarget 2017 Sep 8;8(38):63247-63257

Sakowski SA, Geddes TJ, Thomas DM, Levi E, Hatfield JS, Kuhn DM
Brain research 2006 Apr 26;1085(1):11-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of TPH1 was performed by loading 25 µg of HUVEC (lane 1), HepG2 (lane 2) and NIH-3T3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TPH1 polyclonal antibody (Product # PA1-777) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~51 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of HUVEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Expression and localization of TPH1/2 in rat tissues The expression and localization of TPH1 (A) and TPH2 (B) were analyzed by immunohistochemistry in the rat brain, liver, and kidney of the healthy, depressive model, and treated groups. The images are representative of six independent experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Protein expression of key serotonin synthesis enzymes is upregulated following HSV-1 Infection. (A) Western blot analysis of DDC, TPH-1, and TPH-2 protein expression between HSV-1-infected and mock-infected A549 cell lysates at 24 and 48 hpi. (B) Quantification from A549 cell western blots of relative expression of DDC, TPH-1, and TPH-2 band intensity normalized to beta-actin. (C) Western blot analysis of TPH-2 protein expression between HSV-1-infected and mock-infected HCEC at 24 hpi. (D) Quantification from HCEC western blots of relative expression of TPH-2 band intensity normalized to beta-actin expressed in HCEC cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Herpes simplex virus upregulation of TPH-1 and TPH-2 expression is associated with a corresponding increase in serotonin production. Cells were mock or HSV-1 infected and at 24 h post infection, expression of TPH-2 (top and middle series of panels, alphaTPH2; red), TPH-1, (bottom series of panels, alphaTPH1; red), serotonin (middle and bottom series of panels, alphaSerotonin; green), or HSV-viral proteins (top series of panels, alphaHSV; green) was visualized by confocal immunofluorescent analysis. Cell nuclei were counterstained with DRAQ5, pseudocolored blue and channels merged.

PA1-777