Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX104627 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104627, RRID:AB_1240438
- Product name
- AKR1C3 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- AKR1C3 antibody [C2C3], C-term detects AKR1C3 protein by western blot analysis.A. 50 ?g rat liver lysate/extract 10% SDS-PAGEAKR1C3 antibody [C2C3], C-term (GTX104627) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 10% SDS PAGE GTX104627 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1C3(GTX104627) antibody at 1:200 dilution.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 £gg of AKR1C3 antibody [C2C3], C-term (GTX104627).Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term (GTX104627).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3(GTX104627) antibody at 1:100 dilution.