11194-1-AP

antibody from Invitrogen Antibodies

Targeting: AKR1C3 DDX, HAKRB, HSD17B5, KIAA0119, PGFS

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11194-1-AP

Provider

Invitrogen Antibodies

Product name

AKR1C3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDSKHQCVK LNDGHFMPVL GFGTYAPPEV PRSKALEVTK LAIEAGFRHI DSAHLYNNEE QVGLAIRSKI ADGSVKREDI FYTSKLWSTF HRPELVRPAL ENSLKKAQLD YVDLYLIHSP MSLKPGEELS PTDENGKVIF DIVDLCTTWE AMEKCKDAGL AKSIGVSNFN RRQLEMILNK PGLKYKPVCN QVECHPYFNR SKLLDFCKSK DIVLVAYSAL GSQRDKRWVD PNSPVLLEDP VLCALAKKHK RTPALIALRY QLQRGVVVLA KSYNEQRIRQ NVQVFEFQLT AEDMKAIDGL DRNLHYFNSD SFASHPNYPY SDEY (1-323 aa encoded by BC019230)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11194-1-AP (AKR1C3 antibody) at dilution of 1:100 (under 25x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11194-1-AP (AKR1C3 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11194-1-AP (AKR1C3 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11194-1-AP (AKR1C3 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11194-1-AP (AKR1C3 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AKR1C3 (IP:11194-1-AP, 4ug; Detection:11194-1-AP 1:1000) with HepG2 cells lysate 2400ug.