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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1C3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2, HeLa, AKR1C3shRNA-transfected HeLa, rat liver.
- Concentration
- 1 mg/mL
Submitted references The impact of flutamide on prostaglandin F(2α) synthase and prostaglandin F(2α) receptor expression, and prostaglandin F(2α) concentration in the porcine corpus luteum of pregnancy.
Grzesiak M, Knapczyk-Stwora K, Slomczynska M
Domestic animal endocrinology 2017 Apr;59:81-89
Domestic animal endocrinology 2017 Apr;59:81-89
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AKR1C3 Polyclonal Antibody (Product # PA5-28065). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. AKR1C3 Polyclonal Antibody (Product # PA5-28065) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AKR1C3 Polyclonal Antibody detects AKR1C3 protein by western blot analysis. A. 50 µg rat liver lysate/extract.10% SDS-PAGE. AKR1C3 Polyclonal Antibody (Product # PA5-28065) dilution: 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AKR1C3 in paraformaldehyde-fixed HeLa cells using an AKR1C3 polyclonal antibody (Product # PA5-28065) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using AKR1C3 (Product # PA5-28065) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of AKR1C3 was performed in HepG2 whole cell extracts using 5 µg of AKR1C3 Polyclonal Antibody (Product # PA5-28065). Samples were transferred to a membrane and probed with AKR1C3 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
