OAAB17155

antibody from Aviva Systems Biology

Targeting: FGFR2 BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB17155

Provider

Aviva Systems Biology

Product name

FGFR2 antibody - C - terminal region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of anti-hFGFR2-C808 Pab (Cat. # OAAB17155) in 293 cell line lysates transiently transfected with the FGFR2 gene (2ug/lane). FGFR2 (arrow) was detected using the purified Pab.There is BioGPS gene expression data showing that FGFR2 is expressed in NCI-H460FGFR2 is supported by BioGPS gene expression data to be expressed in HEK293

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

FGFR2 Antibody (C-term) (Cat. # OAAB17155) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.