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LearnMAB6843-100
antibody from R&D Systems
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB6843-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGFR2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects all isoforms of human FGF R2 ( alpha and beta , IIIb and IIIc). In direct ELISAs, approximately 25-50% cross-reactivity with mouse FGF R2 and no cross-reactivity with any isoform of recombinant human (rh) FGF R1, rhFGF R3, or rhFGF R4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 98739
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
Keratinocyte growth factor (KGF) enhances postnatal T-cell development via enhancements in proliferation and function of thymic epithelial cells.
Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
Stem cells (Dayton, Ohio) 2012 Jul;30(7):1455-64
Stem cells (Dayton, Ohio) 2012 Jul;30(7):1455-64
Keratinocyte growth factor (KGF) enhances postnatal T-cell development via enhancements in proliferation and function of thymic epithelial cells.
Rossi SW, Jeker LT, Ueno T, Kuse S, Keller MP, Zuklys S, Gudkov AV, Takahama Y, Krenger W, Blazar BR, Holländer GA
Blood 2007 May 1;109(9):3803-11
Blood 2007 May 1;109(9):3803-11
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FGF R2 in Human Adenocarcinoma. FGF R2 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human FGF R2 Monoclonal Antibody (Catalog # MAB6843) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 beta (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB6843). The ND50 is typically 0.1-0.5 µg/mL in the presence of heparin (10 µg/mL).
