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LearnF50617
antibody from NSJ Bioreagents
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- F50617 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- FGFR2 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 7-37 from the human protein was used as the immunogen for this FGFR2 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the FGFR2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from (1) HeLa, (2) K562 and (3) T47D cell line using FGFR2 antibody at 1:1000. Predicted molecular weight: 80-120 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from (1) HeLa, (2) K562 and (3) MCF-7 cell line using FGFR2 antibody at 1:1000. Predicted molecular weight: 80-120 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FGFR2 antibody western blot analysis in mouse NIH3T3 lysate. Predicted molecular weight: 80-120 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of HeLa cells stained with FGFR2 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain section using FGFR2 antibody; Ab was diluted at 1:25 dilution.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human liver section using FGFR2 antibody; Ab was diluted at 1:25.
