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- Antibody Data
- Antigen structure
- References [14]
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- Western blot [4]
- Immunohistochemistry [5]
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- Product number
- NBP2-15339 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-15339, RRID:AB_2665496
- Product name
- Rabbit Polyclonal ALDH1A3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Hypoxia-inducible factor-1α mediates the expression of mature β cell-disallowed genes in hypoxia-induced β cell dedifferentiation.
Sel1L-Hrd1 ER-associated degradation maintains β cell identity via TGF-β signaling.
Adipsin preserves beta cells in diabetic mice and associates with protection from type 2 diabetes in humans.
Induction of α cell-restricted Gc in dedifferentiating β cells contributes to stress-induced β-cell dysfunction.
Episodic β-cell death and dedifferentiation during diet-induced obesity and dysglycemia in male mice.
Targeting ALDH2 with disulfiram/copper reverses the resistance of cancer cells to microtubule inhibitors.
Insulin-Deficient Mouse β-Cells Do Not Fully Maturebut Can Be Remedied Through Insulin Replacementby Islet Transplantation.
db/db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet.
Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes.
Pair Feeding, but Not Insulin, Phloridzin, or Rosiglitazone Treatment, Curtails Markers of β-Cell Dedifferentiation in db/db Mice.
CDK4/6 inhibition is more active against the glioblastoma proneural subtype.
Evidence of β-Cell Dedifferentiation in Human Type 2 Diabetes.
Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic β cells in diabetic mice.
Characterization of the sebocyte lipid droplet proteome reveals novel potential regulators of sebaceous lipogenesis.
Liu N, Cai X, Liu T, Zou J, Wang L, Wang G, Liu Y, Ding X, Zhang B, Sun P, Liang R, Wang S
Biochemical and biophysical research communications 2020 Mar 5;523(2):382-388
Biochemical and biophysical research communications 2020 Mar 5;523(2):382-388
Sel1L-Hrd1 ER-associated degradation maintains β cell identity via TGF-β signaling.
Shrestha N, Liu T, Ji Y, Reinert RB, Torres M, Li X, Zhang M, Tang CA, Hu CA, Liu C, Naji A, Liu M, Lin JD, Kersten S, Arvan P, Qi L
The Journal of clinical investigation 2020 Jul 1;130(7):3499-3510
The Journal of clinical investigation 2020 Jul 1;130(7):3499-3510
Adipsin preserves beta cells in diabetic mice and associates with protection from type 2 diabetes in humans.
Gómez-Banoy N, Guseh JS, Li G, Rubio-Navarro A, Chen T, Poirier B, Putzel G, Rosselot C, Pabón MA, Camporez JP, Bhambhani V, Hwang SJ, Yao C, Perry RJ, Mukherjee S, Larson MG, Levy D, Dow LE, Shulman GI, Dephoure N, Garcia-Ocana A, Hao M, Spiegelman BM, Ho JE, Lo JC
Nature medicine 2019 Nov;25(11):1739-1747
Nature medicine 2019 Nov;25(11):1739-1747
Induction of α cell-restricted Gc in dedifferentiating β cells contributes to stress-induced β-cell dysfunction.
Kuo T, Damle M, González BJ, Egli D, Lazar MA, Accili D
JCI insight 2019 May 23;5(13)
JCI insight 2019 May 23;5(13)
Episodic β-cell death and dedifferentiation during diet-induced obesity and dysglycemia in male mice.
Tersey SA, Levasseur EM, Syed F, Farb TB, Orr KS, Nelson JB, Shaw JL, Bokvist K, Mather KJ, Mirmira RG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May 29;32(11):fj201800150RR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May 29;32(11):fj201800150RR
Targeting ALDH2 with disulfiram/copper reverses the resistance of cancer cells to microtubule inhibitors.
Wang NN, Wang LH, Li Y, Fu SY, Xue X, Jia LN, Yuan XZ, Wang YT, Tang X, Yang JY, Wu CF
Experimental cell research 2018 Jan 1;362(1):72-82
Experimental cell research 2018 Jan 1;362(1):72-82
Insulin-Deficient Mouse β-Cells Do Not Fully Maturebut Can Be Remedied Through Insulin Replacementby Islet Transplantation.
Ramzy A, Mojibian M, Kieffer TJ
Endocrinology 2018 Jan 1;159(1):83-102
Endocrinology 2018 Jan 1;159(1):83-102
db/db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet.
Burke SJ, Batdorf HM, Burk DH, Noland RC, Eder AE, Boulos MS, Karlstad MD, Collier JJ
Journal of diabetes research 2017;2017:8503754
Journal of diabetes research 2017;2017:8503754
Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes.
Feng AL, Xiang YY, Gui L, Kaltsidis G, Feng Q, Lu WY
Diabetologia 2017 Jun;60(6):1033-1042
Diabetologia 2017 Jun;60(6):1033-1042
Pair Feeding, but Not Insulin, Phloridzin, or Rosiglitazone Treatment, Curtails Markers of β-Cell Dedifferentiation in db/db Mice.
Ishida E, Kim-Muller JY, Accili D
Diabetes 2017 Aug;66(8):2092-2101
Diabetes 2017 Aug;66(8):2092-2101
CDK4/6 inhibition is more active against the glioblastoma proneural subtype.
Li M, Xiao A, Floyd D, Olmez I, Lee J, Godlewski J, Bronisz A, Bhat KPL, Sulman EP, Nakano I, Purow B
Oncotarget 2017 Aug 15;8(33):55319-55331
Oncotarget 2017 Aug 15;8(33):55319-55331
Evidence of β-Cell Dedifferentiation in Human Type 2 Diabetes.
Cinti F, Bouchi R, Kim-Muller JY, Ohmura Y, Sandoval PR, Masini M, Marselli L, Suleiman M, Ratner LE, Marchetti P, Accili D
The Journal of clinical endocrinology and metabolism 2016 Mar;101(3):1044-54
The Journal of clinical endocrinology and metabolism 2016 Mar;101(3):1044-54
Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic β cells in diabetic mice.
Kim-Muller JY, Fan J, Kim YJ, Lee SA, Ishida E, Blaner WS, Accili D
Nature communications 2016 Aug 30;7:12631
Nature communications 2016 Aug 30;7:12631
Characterization of the sebocyte lipid droplet proteome reveals novel potential regulators of sebaceous lipogenesis.
Dahlhoff M, Fröhlich T, Arnold GJ, Müller U, Leonhardt H, Zouboulis CC, Schneider MR
Experimental cell research 2015 Mar 1;332(1):146-55
Experimental cell research 2015 Mar 1;332(1):146-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH1A3 Antibody [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) antibodies. Blue arrows indicate insulin+Aldh1A3+ cells. IHC-Fr image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ALDH1A3 Antibody [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/jdr/2017/8503754/) licensed under a CC-BY licence.
