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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX110784 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110784, RRID:AB_10727704
- Product name
- ALDH1A3 antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references ALDH1A3, the Major Aldehyde Dehydrogenase Isoform in Human Cholangiocarcinoma Cells, Affects Prognosis and Gemcitabine Resistance in Cholangiocarcinoma Patients.
Chen MH, Weng JJ, Cheng CT, Wu RC, Huang SC, Wu CE, Chung YH, Liu CY, Chang MH, Chen MH, Chiang KC, Yeh TS, Su Y, Yeh CN
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Aug 15;22(16):4225-35
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Aug 15;22(16):4225-35
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 7.5% SDS PAGE GTX110784 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A549, using ALDH1A3(GTX110784) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10£gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ALDH1A3(GTX110784) antibody at 1:500 dilution.
