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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA502841 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502841, RRID:AB_2622384
- Product name
- ALDH1A3 mouse monoclonal antibody, clone OTI4E8 (formerly 4E8)
- Antibody type
- Monoclonal
- Description
- ALDH1A3 mouse monoclonal antibody, clone OTI4E8 (formerly 4E8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ALDH1A3
- Isotype
- IgG
- Antibody clone number
- OTI4E8
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Establishment of MAGEC2-knockout cells and functional investigation of MAGEC2 in tumor cells.
Wang J, Song X, Guo C, Wang Y, Yin Y
Cancer science 2016 Dec;107(12):1888-1897
Cancer science 2016 Dec;107(12):1888-1897
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1A3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human ALDH1A3 (NP_000648) gene (the fusion of amino acids 1-100 and 413-512) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted with anti-ALDH1A3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ALDH1A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA502841)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALDH1A3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA502841)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ALDH1A3 antibody(TA502841),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
