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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-27727 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH1A3 Monoclonal Antibody (GT926)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A431, Huh-7, U87-MG, SK-N-AS
- Antibody clone number
- GT926
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH1A3 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with ALDH1A3 monoclonal antibody (Product # MA5-27727) using a dilution of 1:1000 followed by HRP-conjugated anti-mouse IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH1A3 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with ALDH1A3 monoclonal antibody (Product # MA5-27727) using a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH1A3 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with ALDH1A3 monoclonal antibody (Product # MA5-27727) using a dilution of 1:1000 followed by HRP-conjugated anti-mouse IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH1A3 Monoclonal Antibody (GT926) detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 protein stained by ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALDH1A3 was performed in paraffin-embedded mouse prostate tissue using ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALDH1A3 was performed in paraffin-embedded rat prostate tissue using ALDH1A3 Monoclonal Antibody (GT926) (Product # MA5-27727) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
