NB300-222

antibody from Novus Biologicals

Targeting: NEFM NEF3, NF-M, NFM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-222

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-222, RRID:AB_10002431

Product name

Chicken Polyclonal NF-M Antibody

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation.

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Porcine

Host

Chicken/Avian

Isotype

IgY

Vial size

0.25 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Muscleblind acts as a modifier of FUS toxicity by modulating stress granule dynamics and SMN localization.

Casci I, Krishnamurthy K, Kour S, Tripathy V, Ramesh N, Anderson EN, Marrone L, Grant RA, Oliver S, Gochenaur L, Patel K, Sterneckert J, Gleixner AM, Donnelly CJ, Ruepp MD, Sini AM, Zuccaro E, Pennuto M, Pasinelli P, Pandey UB
Nature communications 2019 Dec 6;10(1):5583

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Axonal and myelinic pathology in 5xFAD Alzheimer's mouse spinal cord.

Chu TH, Cummins K, Sparling JS, Tsutsui S, Brideau C, Nilsson KPR, Joseph JT, Stys PK
PloS one 2017;12(11):e0188218

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Tumor suppressor menin is required for subunit-specific nAChR α5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons.

Getz AM, Xu F, Visser F, Persson R, Syed NI
Scientific reports 2017 May 11;7(1):1768

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The mitochondrial division inhibitor Mdivi-1 rescues mammalian neurons from anesthetic-induced cytotoxicity.

Xu F, Armstrong R, Urrego D, Qazzaz M, Pehar M, Armstrong JN, Shutt T, Syed N
Molecular brain 2016 Mar 24;9:35

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Mechanisms of action of naturally occurring antibodies against β-amyloid on microglia.

Gold M, Mengel D, Röskam S, Dodel R, Bach JP
Journal of neuroinflammation 2013 Jan 14;10:5

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Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes.

Portmann-Lanz CB, Schoeberlein A, Portmann R, Mohr S, Rollini P, Sager R, Surbek DV
American journal of obstetrics and gynecology 2010 Mar;202(3):294.e1-294.e11

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.

Other assay

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.