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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- T2551 - Provider product page
- Provider
- MilliporeSigma / Merck KGaA
- Product name
- Monoclonal Anti-Tenascin antibody produced in mouse
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- BC-24
- Storage
- Store at -20°C
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Theoretical target weight
- [kDa] 148
- Lane 6
- Tonsil
- Lane 5
- Liver
- Lane 4
- Human Plasma
- Lane 3
- U-251MG sp
- Lane 2
- RT-4
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human liver shows strong cytoplasmic positivity in bile duct cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
- Submitted by
- 56b9f9be53f2b
- Main image
- Experimental details
- Antigen retreival with pepsin (Sigma, cat no. R2283) at 37 degrees centigrade for 10 minutes. Endogenous peroxidase block, normal horse serum block, secondary antibody and chromogen from Vector Anti-Mouse ImmPRESS Excel kit (Vector, cat no. MP-7602). Primary antibody made up in 1xTBS with 2.5% normal horse serum.
- Sample type
- formalin fixed, paraffin embedded bronchial biopsy
- Validation comment
- Immunohistochemistry FFPE tissue
- Primary Ab dilution
- 1 in 4000
- Other comments
- Excellent staining especially in the basement membrane.
- Conjugate
- Unconjugated
- Secondary Ab dilution
- RTU from kit, dropper.
