Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [2]
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Validation data
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- Product number
- STJ70260 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-BOB1/OCAB/OBF1 antibody (C-Term) (STJ70260)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-BOB1/OCAB/OBF1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
DAYALNHTLSVEGF
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70260 (0. 3µg/ml) staining of Human (A) , Mouse (B) and Rat (C) Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human BOB1 with DYKDDDDK tag probed with STJ70260 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with STJ70260 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA