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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 5450-MSM4-P0 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BOB.1 Monoclonal Antibody (BOB1, 2424)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BOB1, 2424
- Vial size
- 20 µg
- Concentration
- 200 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Raji cell lysate using BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Raji cell lysate using BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed Raji cells using BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human spleen using BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of paraformaldehyde-fixed Raji cells using BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Analysis of Protein Array containing more than 19,000 full-length human proteins BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-PAGE Analyis Purified BOB.1 Monoclonal Antibody (Product # 5450-MSM4-P1). Confirmation of Purity and Integrity of Antibody.
