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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [3]
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- Product number
- PA5-26413 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p19 INK4d Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Differential expression of cell cycle regulators in CDK5-dependent medullary thyroid carcinoma tumorigenesis.
Pozo K, Hillmann A, Augustyn A, Plattner F, Hai T, Singh T, Ramezani S, Sun X, Pfragner R, Minna JD, Cote GJ, Chen H, Bibb JA, Nwariaku FE
Oncotarget 2015 May 20;6(14):12080-93
Oncotarget 2015 May 20;6(14):12080-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a p19 polyclonal antibody (Product # PA5-26413) in mouse testis tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded testis tissue using a p19 polyclonal antibody (Product # PA5-26413), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a p19 polyclonal antibody (Product # PA5-26413) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Evaluation of the effects of p25-GFP overexpression on cell cycle protein expression in malignant versus benign mouse MTC Immunoblots of lysates from proliferating malignant (P) and arrested benign (A) mouse MTC for A ) CDK2, CDK4 and CDK6; B ) cyclins A1, B1, D1, E2 and C ) p15 INK4b , p16 INK4a , p18 INK4c , p19 INK4d , p21 CIP/WAF1 and p27 KIP . P-values are p < 0.0001 for CDK2, p = 0.6974 for CDK4, p = 0.0633 for CDK6; p < 0.0001 for cyclin-A1, p = 0.0194 for cyclin-B1, p = 0.3416 for cyclin-D1, p < 0.0001 for cyclin-E2; p = 0.0002 for p15 INK4b , p = 0.291 for p16 INK4a , p = 0.0006 for p18 INK4c , p = 0.0054 for p19 INK4d , p = 0.02 for p21 CIP/WAF and p = 0.67 for p27 KIP1 . Data are represented as mean +/- SEM, N = 6 for each condition.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Evaluation of the effect of CDK5 activity on cell cycle protein expression in human MTC cells Immunoblots of lysates from MTC-SK cells transfected with either a control plasmid (Con) or a kinase-dead (Kd) CDK5 encoding plasmid for A ) CDK2, CDK4 and CDK6; B ) cyclin-A1,-B1,-E2 and C ) p15 INK4b , p16 INK4a , p18 INK4c , p19 INK4d , p21 CIP/WAF1 and p27 KIP . P-values are p = 0.0244 for CDK2, p = 0.038 for CDK4, p = 0.009 for CDK6; p < 0.0001 for cyclin-A1, p = 0.0005 for cyclin-B1, p = 0.0116 for cyclin-E2; p = 0.0044 for p15 INK4b , p = 0.4687 for p16 INK4a , p = 0.1032 for p18 INK4c , p = 0.0195 for p19 INK4d , p = 0.0022 for p21 CIP/WAF and p = 0.2674 for p27 KIP1 . Data are represented as mean +/- SEM, N = 4-6 for each condition.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Analysis of cell cycle protein expression in human MTC samples Representative immunoblots of lysates from control thyroid tissue (Co), sporadic (Sp) and hereditary (H) human MTC tumors with antibodies as indicated are shown with quantification. Protein levels were normalized to Coomassie blue (CB) signal. Immunoblots were probed with antibodies to A ) CDK2, CDK4 and CDK6; B ) cyclins-A1, -D1, -E2; C ) in p15 INK4b , p16 INK4a , p18 INK4c , p19 INK4d , p21 CIP/WAF1 and p27 KIP . P-values were for CDK2, Sp, p = 0.0168 and H, p = 0.0140; for CDK4, Sp, p = 0.4072 and H, p = 0.8576; for CDK6, Sp, p = 0.5484 and H, p = 0.4916; for cyclin-A1, Sp, p = 0.9923 and H, p = 0.7017; for cyclin-D1, Sp, p = 0.0307 and H, p = 0.6883; for cyclin-E2, Sp, p = 0.2645 and H, p = 0.3070; for p15 INK4b , Sp, p = 0.0088 and H, p = 0.0310; for p16 INK4a , Sp, p = 0.0014 and H, p = 0.0496; for p18 INK4c , Sp, p = 0.0309 and H, p = 0.1054; for p19 INK4d , Sp, p = 0.0484 and H, p = 0.3560; for p21 CIP/WAF , Sp, p = 0.0554 and H, p = 0.6807; for p27 KIP1 , Sp, p = 0.2149 and H, p = 0.2131. Data are represented as mean +/- SEM, N = 4 for each condition.
