PA5-31815

antibody from Invitrogen Antibodies

Targeting: ZNF711 CMPX1, dJ75N13.1, MRX65, MRX97, Zfp711, ZNF4, ZNF5, ZNF6

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31815

Provider

Invitrogen Antibodies

Product name

ZNF711 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: SK-N-SH, SK-N-SH nuclear extract, ZNF711 transfected 293T lysate.

Concentration

1 mg/mL

References

Submitted references

Characterization of the ZFX family of transcription factors that bind downstream of the start site of CpG island promoters.

Ni W, Perez AA, Schreiner S, Nicolet CM, Farnham PJ
Nucleic acids research 2020 Jun 19;48(11):5986-6000

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ZNF711 was performed by separating 30 µg of SK-N-SH whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZNF711 Polyclonal Antibody (Product # PA5-31815) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ZNF711 Polyclonal Antibody (Product # PA5-31815). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysates. Lane B: ZNF711 transfected 293T lysate . 7.5% SDS PAGE. ZNF711 Polyclonal Antibody (Product # PA5-31815) diluted at 1:5,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. Characterization of ZFX and ZNF711 binding sites. ( A ) Classification of binding sites based on genomic locations of all ZFX peaks located downstream of the TSS and ZFX downstream peaks at promoters of genes down-regulated in all 3 DKO clones. ( B ) Classification of ZNF711 downstream ChIP-seq peaks, downstream peaks identified using read2 of the ChIP-exo dataset, and downstream peaks identified by the ChexMix program (+/-10 nt from the nt identified as the binding site by the program). ( C ) Tag density plots of all ZNF711 peaks from standard ChIP-seq and from ChIP-exo. ( D ) Motif analysis using the top 5000 peaks identified from standard ZNF711 ChIP-seq (average width 1800 nt), ChIP-exo read2 (average width 300 nt), ChIP-exo by the ChexMix program (20 nt), and randomized CpG island promoter regions (width 2 kb, 200 bp, and 20 nt). The peaks were searched for the known ZNF711 motif and the 5 nt motif identified by ChIP-exo. ( E) Zoom-in comparison of peaks from ZNF711 standard ChIP-seq replicates and ChIP-exo replicates.