NB600-1335

Product number: NB600-1335 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB600-1335, RRID:AB_2089251
Product name: Mouse Monoclonal GADD153/CHOP Antibody
Antibody type: Monoclonal
Description: Protein A purified.
Reactivity: Human, Mouse, Rat, Simian
Host: Mouse
Antigen sequence: The N-terminal region of CHOP/GADD1 53.
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

DDIT3 protein structure - NB600-1335 shown in red.

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Cannabidiol Enhances the Therapeutic Effects of TRAIL by Upregulating DR5 in Colorectal Cancer.

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Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresponsiveness by inhibiting UPR transducers.

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Short-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice.

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A FBN1 3'UTR mutation variant is associated with endoplasmic reticulum stress in aortic aneurysm in Marfan syndrome.

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A Novel Animal Model for Studying Depression Featuring the Induction of the Unfolded Protein Response in Hippocampus.

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Gingival Periodontal Disease (PD) Level-Butyric Acid Affects the Systemic Blood and Brain Organ: Insights Into the Systemic Inflammation of Periodontal Disease.

Cueno ME, Ochiai K
Frontiers in immunology 2018;9:1158

Placental hypoxia inducible factor -1α & CHOP immuno-histochemical expression relative to maternal circulatory syncytiotrophoblast micro-vesicles in preeclamptic and normotensive pregnancies.

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Piperine ameliorates SCA17 neuropathology by reducing ER stress.

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HIF-1α triggers ER stress and CHOP-mediated apoptosis in alveolar epithelial cells, a key event in pulmonary fibrosis.

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American journal of physiology. Gastrointestinal and liver physiology 2015 May 1;308(9):G757-66

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The autophagy-related marker LC3 can predict prognosis in human hepatocellular carcinoma.

Lee YJ, Hah YJ, Kang YN, Kang KJ, Hwang JS, Chung WJ, Cho KB, Park KS, Kim ES, Seo HY, Kim MK, Park KG, Jang BK
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Lin CJ, Lee CC, Shih YL, Lin CH, Wang SH, Chen TH, Shih CM
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CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI.

Sok J, Wang XZ, Batchvarova N, Kuroda M, Harding H, Ron D
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Sok J, Wang XZ, Batchvarova N, Kuroda M, Harding H, Ron D
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Wang XZ, Harding HP, Zhang Y, Jolicoeur EM, Kuroda M, Ron D
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Wang XZ, Kuroda M, Sok J, Batchvarova N, Kimmel R, Chung P, Zinszner H, Ron D
The EMBO journal 1998 Jul 1;17(13):3619-30

Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins.

Saisanit S, Sun XH
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Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins.

Saisanit S, Sun XH
Molecular and cellular biology 1997 Feb;17(2):844-50

TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling.

Zinszner H, Sok J, Immanuel D, Yin Y, Ron D
Journal of cell science 1997 Aug;110 ( Pt 15):1741-50

Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase.

Wang XZ, Ron D
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Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase.

Wang XZ, Ron D
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Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153).

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Molecular and cellular biology 1996 Aug;16(8):4273-80

Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153).

Wang XZ, Lawson B, Brewer JW, Zinszner H, Sanjay A, Mi LJ, Boorstein R, Kreibich G, Hendershot LM, Ron D
Molecular and cellular biology 1996 Aug;16(8):4273-80

Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153).

Batchvarova N, Wang XZ, Ron D
The EMBO journal 1995 Oct 2;14(19):4654-61

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.