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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB7224 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GADD153 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GADD153 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P35638- Isotype
- IgG
- Antibody clone number
- 671708
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis.
Zhang L, Tian Z, Li W, Wang X, Man Z, Sun S
Bioscience reports 2017 Jul 31;
Bioscience reports 2017 Jul 31;
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- GADD153 in Jurkat Human Cell Line. GADD153 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with PMA and hydrogen peroxide using Mouse Anti-Human GADD153 Monoclonal Antibody (Catalog # MAB7224) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
