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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- APR-031-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAR1 (F2R) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human colon cancer HT-29 (lanes 1 and 3) and human Colo-205 colon cancer (lanes 2 and 4) cell line lysates: - 1,2. Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:200).3,4. Anti-Human PAR1 (F2R) (extracellular) Antibody , preincubated with Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human colon cancer HT-29 (lanes 1 and 3) and human Colo-205 colon cancer (lanes 2 and 4) cell line lysates: - 1,2. Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:200).3,4. Anti-Human PAR1 (F2R) (extracellular) Antibody , preincubated with Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human promyelocytic leukemia HL-60 (lanes 1 and 4), human T-cell leukemia Jurkat (lanes 2 and 5), and chronic myelogenous leukemia K562 (lanes 3 and 6) cell line lysates: - 1-3. Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:200).4-6. Anti-Human PAR1 (F2R) (extracellular) Antibody , preincubated with Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of PAR-1 in human prostate PC-3 cell line - Cell surface detection of PAR-1 in human prostate PC-3 cell line. A.Live intact PC-3 cells were stained with Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:50), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red staining). Nuclei were visualized using the cell-permeable dye Hoechst 33342 (blue). B. Live view of the same field as A.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of PAR-1 in human prostate PC-3 cell line - Cell surface detection of PAR-1 in human prostate PC-3 cell line. A.Live intact PC-3 cells were stained with Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:50), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red staining). Nuclei were visualized using the cell-permeable dye Hoechst 33342 (blue). B. Live view of the same field as A.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of PAR-1 in normal human breast and human breast carcinoma - Immunohistochemical staining of paraffin-embedded human breast sections using Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:100).PAR-1 staining is highly specific for epithelium-derived cells. A. In the normal resting breast, epithelial cells of the mammary ducts are visible using Histofine (pink).B.The breast carcinoma contains epithelium-derived malignant cells stained with DAB (brown).Hematoxilinis used as the counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of PAR-1 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (2.5μg) + goat- Anti-rabbit-FITC.
