Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB3855 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PAR1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human PAR1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P25116- Isotype
- IgG
- Antibody clone number
- 731115
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Data regarding the effects of thrombin and dabigatran-inhibited thrombin on protease-activated receptor 1 and activation of human atrial fibroblasts.
Altieri P, Bertolotto M, Fabbi P, Sportelli E, Balbi M, Santini F, Brunelli C, Canepa M, Montecucco F, Ameri P
Data in brief 2018 Aug;19:925-931
Data in brief 2018 Aug;19:925-931
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PAR1 by Western Blot. Western blot shows lysates of Y-79 human retinoblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAR1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PAR1 in Human Colon Cancer Tissue. PAR1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of PAR1 in Human Peripheral Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of PAR1 in HT-29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 Monoclonal Antibody (Catalog # MAB3855, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
