Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- M01253 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cortactin CTTN Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for Cortactin/CTTN detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC-P, ICC, WB. Reactive species: Human, Mouse, Rat Cortactin/CTTN information: Molecular Weight: 61586 MW; Subcellular Localization: Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Cell projection, ruffle. Cell projection, dendrite . Cell projection . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, podosome . Cell junction . Cell junction, focal adhesion . Membrane, clathrin-coated pit . Cell projection, dendritic spine . Cytoplasm, cell cortex . Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- FAI-3
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Systematic simulation of tumor cell invasion and migration in response to time-varying rotating magnetic field.
Zhang S, Yu T, Zhang G, Chen M, Yin D, Zhang C
Biomechanics and modeling in mechanobiology 2024 May 27;
Biomechanics and modeling in mechanobiology 2024 May 27;
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of Cortactin expression in HeLa cell lysate (M01253). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTTN monoclonal antibody (Catalog # M01253) overnight at 4
- Additional image