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- Antibody Data
- Antigen structure
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- Validations
- ELISA [2]
- Flow cytometry [1]
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- Product number
- Ab175859 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Gemtuzumab (anti-CD33)
- Antibody type
- Recombinant
- Description
- Gemtuzumab (anti-CD33) is a monoclonal antibody targeting CD33 antigen, which present on leukemic myeloblasts of acute myeloid leukemia (AML). Gemtuzumab (anti-CD33) can be used for synthesis of antibody-drug conjugate (ADC), Gemtuzumab ozogamicin. Gemtuzumab ozogamicin consists of a cytotoxic derivative of Calicheamicin (a cytotoxic antibiotic), and a monoclonal antibody. Gemtuzumab ozogamicin can be used for the research of acute myeloid leukemia.Purity>95% (SDS-PAGE&SEC)Endotoxin Level< 1.0EU/mg
- Reactivity
- Human
- Conjugate
- Unconjugated
- Vial size
- 100_g,10mg,1mg,5mg
- Concentration
- Lot by Lot
- Storage
- Store at -80_ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Gemtuzumab (anti-CD33) (Ab175859) - ELISA Immobilized Recombinant Human CD33 Protein (rp143966) at 2.0 μg/mL can bind Gemtuzumab (anti-CD33) (Ab175859). The ED₅₀ for this effect is 391.3 ng/mL.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Gemtuzumab (anti-CD33) (Ab175859) - ELISA Immobilized Recombinant Human Siglec-3/CD33 Protein (rp169578) at 1.0 μg/mL can bind Gemtuzumab (anti-CD33) (Ab175859) with the EC₅₀ of 36.93 ng/mL.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Gemtuzumab (anti-CD33) (Ab175859) - Flow Cytometry Flow Cytometry analysis of THP-1 cells labelling CD33 (red) with Gemtuzumab (anti-CD33) (Ab175859). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG4SP. Black - Unlabelled control, cells without incubation with primary antibody.
