V4637

antibody from NSJ Bioreagents

Targeting: CD33 FLJ00391, p67, SIGLEC-3, SIGLEC3

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V4637

Provider

NSJ Bioreagents

Product name

CD33 Antibody

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

SIGLEC3/3597

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the SIGLEC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lymph node tissue with SIGLEC3 antibody (clone SIGLEC3/3597). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human spleen tissue with SIGLEC3 antibody (clone SIGLEC3/3597). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using SIGLEC3 antibody (clone SIGLEC3/3597). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free SIGLEC3 antibody (clone SIGLEC3/3597) as confirmation of integrity and purity.