ARP30799_P050
antibody from Aviva Systems Biology
Targeting: CXCR4
CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- ARP30799_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP30799_P050, RRID:AB_2045328
- Product name
- CXCR4 antibody - N-terminal region (ARP30799_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the N terminal region of human CXCR4
- Description
- Affinity Purified
- Reactivity
- Human, Mouse, Rat, Horse, Porcine, Rabbit
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- CXCR4 antibody - N-terminal region (ARP30799_P050) validated by WB using 721_B cell Lysate at 0.2-1 ug/ml,.
- Sample type
- 721_B cell Lysate
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- CXCR4 antibody - N-terminal region (ARP30799_P050) validated by WB using human microvascular endothelial cells (25ug) at 1:1000.
- Sample type
- human microvascular endothelial cells (25ug)
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- WB Suggested Anti-CXCR4 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: 721_B cell lysate
- Sample type
- HMEC-1 and A549 cells
- Primary Ab dilution
- 1:100
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry with HMEC-1 and A549 cells tissue
- Sample type
- HMEC-1 and A549 cells
- Primary Ab dilution
- 0.2-1 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type: human colon tissues infected ex-vivo with HIV-1 Green: Primary Blue: DAPI Primary Dilution: 1:100 Secondary Antibody: Donkey anti-Rabbit AF 488 Secondary Dilution: 1:500 Image Submitted By: Chiara Foglieni San Raffaele Scientific Institute, Milan, Italy
- Sample type
- HMEC-1 and A549 cells
- Primary Ab dilution
- 0.2-1 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type: HMEC-1 and A549 cells