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LearnMAB172-100
antibody from R&D Systems
Targeting: CXCR4
CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [38]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB172-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 44716
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy.
Senescent fibroblasts drive ageing pigmentation: A potential therapeutic target for senile lentigo.
Breast Cancer: An Examination of the Potential of ACKR3 to Modify the Response of CXCR4 to CXCL12.
Tumor microenvironment in functional adrenocortical adenomas: immune cell infiltration in cortisol-producing adrenocortical adenoma.
Expression of CXC-motif-chemokine 12 and the receptor C-X-C receptor 4 in glioma and theeffect on peritumoral brain edema.
Mechanisms by which CXCR4/CXCL12 cause metastatic behavior in pancreatic cancer.
Cathepsin K cleavage of SDF-1α inhibits its chemotactic activity towards glioblastoma stem-like cells.
Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth.
Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression.
Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
The Glycosylphosphatidylinositol-Anchored Variable Region of Llama Heavy Chain-Only Antibody JM4 Efficiently Blocks both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1.
Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices.
The long pentraxin PTX3 promotes fibrocyte differentiation.
Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Expression of the chemokine receptor CXCR7 in CXCR4-expressing human 143B osteosarcoma cells enhances lung metastasis of intratibial xenografts in SCID mice.
The expressions of MIF and CXCR4 protein in tumor microenvironment are adverse prognostic factors in patients with esophageal squamous cell carcinoma.
CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.
The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
Alternative implication of CXCR4 in JAK2/STAT3 activation in small cell lung cancer.
A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
Clinical and biological significance of CXCL12 and CXCR4 expression in adult testes and germ cell tumours of adults and adolescents.
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
Dynamic stromal-epithelial interactions during progression of MCF10DCIS.com xenografts.
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF.
Human melanoma metastases express functional CXCR4.
Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells.
CXCR4 antagonist inhibits stromal cell-derived factor 1-induced migration and invasion of human pancreatic cancer.
CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
CXCR4 function requires membrane cholesterol: implications for HIV infection.
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Giampieri R, Piva F, Occhipinti G, Bittoni A, Righetti A, Pagliaretta S, Murrone A, Bianchi F, Amantini C, Giulietti M, Ricci G, Principato G, Santoni G, Berardi R, Cascinu S
PloS one 2019;14(5):e0215990
PloS one 2019;14(5):e0215990
Senescent fibroblasts drive ageing pigmentation: A potential therapeutic target for senile lentigo.
Yoon JE, Kim Y, Kwon S, Kim M, Kim YH, Kim JH, Park TJ, Kang HY
Theranostics 2018;8(17):4620-4632
Theranostics 2018;8(17):4620-4632
Breast Cancer: An Examination of the Potential of ACKR3 to Modify the Response of CXCR4 to CXCL12.
Del Molino Del Barrio I, Wilkins GC, Meeson A, Ali S, Kirby JA
International journal of molecular sciences 2018 Nov 14;19(11)
International journal of molecular sciences 2018 Nov 14;19(11)
Tumor microenvironment in functional adrenocortical adenomas: immune cell infiltration in cortisol-producing adrenocortical adenoma.
Kitawaki Y, Nakamura Y, Kubota-Nakayama F, Yamazaki Y, Miki Y, Hata S, Ise K, Kikuchi K, Morimoto R, Satoh F, Sasano H
Human pathology 2018 Jul;77:88-97
Human pathology 2018 Jul;77:88-97
Expression of CXC-motif-chemokine 12 and the receptor C-X-C receptor 4 in glioma and theeffect on peritumoral brain edema.
Tang W, Chen Y, Wang X, Chen Y, Zhang J, Lin Z
Oncology letters 2018 Feb;15(2):2501-2507
Oncology letters 2018 Feb;15(2):2501-2507
Mechanisms by which CXCR4/CXCL12 cause metastatic behavior in pancreatic cancer.
Zhang J, Liu C, Mo X, Shi H, Li S
Oncology letters 2018 Feb;15(2):1771-1776
Oncology letters 2018 Feb;15(2):1771-1776
Cathepsin K cleavage of SDF-1α inhibits its chemotactic activity towards glioblastoma stem-like cells.
Hira VV, Verbovšek U, Breznik B, Srdič M, Novinec M, Kakar H, Wormer J, der Swaan BV, Lenarčič B, Juliano L, Mehta S, Van Noorden CJ, Lah TT
Biochimica et biophysica acta. Molecular cell research 2017 Mar;1864(3):594-603
Biochimica et biophysica acta. Molecular cell research 2017 Mar;1864(3):594-603
Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth.
Fareh M, Almairac F, Turchi L, Burel-Vandenbos F, Paquis P, Fontaine D, Lacas-Gervais S, Junier MP, Chneiweiss H, Virolle T
Cell death & disease 2017 Mar 30;8(3):e2713
Cell death & disease 2017 Mar 30;8(3):e2713
Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression.
Coke CJ, Scarlett KA, Chetram MA, Jones KJ, Sandifer BJ, Davis AS, Marcus AI, Hinton CV
The Journal of biological chemistry 2016 May 6;291(19):9991-10005
The Journal of biological chemistry 2016 May 6;291(19):9991-10005
Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
Mercurio L, Ajmone-Cat MA, Cecchetti S, Ricci A, Bozzuto G, Molinari A, Manni I, Pollo B, Scala S, Carpinelli G, Minghetti L
Journal of experimental & clinical cancer research : CR 2016 Mar 25;35:55
Journal of experimental & clinical cancer research : CR 2016 Mar 25;35:55
The Glycosylphosphatidylinositol-Anchored Variable Region of Llama Heavy Chain-Only Antibody JM4 Efficiently Blocks both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1.
Liu L, Wang W, Matz J, Ye C, Bracq L, Delon J, Kimata JT, Chen Z, Benichou S, Zhou P
Journal of virology 2016 Dec 1;90(23):10642-10659
Journal of virology 2016 Dec 1;90(23):10642-10659
Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices.
Wescott MP, Kufareva I, Paes C, Goodman JR, Thaker Y, Puffer BA, Berdougo E, Rucker JB, Handel TM, Doranz BJ
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):9928-33
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):9928-33
The long pentraxin PTX3 promotes fibrocyte differentiation.
Pilling D, Cox N, Vakil V, Verbeek JS, Gomer RH
PloS one 2015;10(3):e0119709
PloS one 2015;10(3):e0119709
Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
Jarajapu YP, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter SK, Raizada MK, Boulton ME, Grant MB
PloS one 2014;9(4):e93965
PloS one 2014;9(4):e93965
An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
Zgraggen S, Huggenberger R, Kerl K, Detmar M
PloS one 2014;9(4):e93665
PloS one 2014;9(4):e93665
Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
Yin X, Johns SC, Kim D, Mikulski Z, Salanga CL, Handel TM, Macal M, Zúñiga EI, Fuster MM
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2133-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2133-42
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Hartmann N, Giese NA, Giese T, Poschke I, Offringa R, Werner J, Ryschich E
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Expression of the chemokine receptor CXCR7 in CXCR4-expressing human 143B osteosarcoma cells enhances lung metastasis of intratibial xenografts in SCID mice.
Brennecke P, Arlt MJ, Muff R, Campanile C, Gvozdenovic A, Husmann K, Holzwarth N, Cameroni E, Ehrensperger F, Thelen M, Born W, Fuchs B
PloS one 2013;8(9):e74045
PloS one 2013;8(9):e74045
The expressions of MIF and CXCR4 protein in tumor microenvironment are adverse prognostic factors in patients with esophageal squamous cell carcinoma.
Zhang L, Ye SB, Ma G, Tang XF, Chen SP, He J, Liu WL, Xie D, Zeng YX, Li J
Journal of translational medicine 2013 Mar 8;11:60
Journal of translational medicine 2013 Mar 8;11:60
CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.
Gassenmaier M, Chen D, Buchner A, Henkel L, Schiemann M, Mack B, Schendel DJ, Zimmermann W, Pohla H
Stem cells (Dayton, Ohio) 2013 Aug;31(8):1467-76
Stem cells (Dayton, Ohio) 2013 Aug;31(8):1467-76
The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Jun 1;17(11):3619-30
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Jun 1;17(11):3619-30
Alternative implication of CXCR4 in JAK2/STAT3 activation in small cell lung cancer.
Pfeiffer M, Hartmann TN, Leick M, Catusse J, Schmitt-Graeff A, Burger M
British journal of cancer 2009 Jun 16;100(12):1949-56
British journal of cancer 2009 Jun 16;100(12):1949-56
A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
Developmental biology 2009 Jun 15;330(2):286-304
Developmental biology 2009 Jun 15;330(2):286-304
Clinical and biological significance of CXCL12 and CXCR4 expression in adult testes and germ cell tumours of adults and adolescents.
Gilbert DC, Chandler I, McIntyre A, Goddard NC, Gabe R, Huddart RA, Shipley J
The Journal of pathology 2009 Jan;217(1):94-102
The Journal of pathology 2009 Jan;217(1):94-102
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadière C, Mami-Chouaib F
Cancer research 2009 Aug 1;69(15):6249-55
Cancer research 2009 Aug 1;69(15):6249-55
Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
Cancer research 2008 Mar 15;68(6):1889-95
Cancer research 2008 Mar 15;68(6):1889-95
Dynamic stromal-epithelial interactions during progression of MCF10DCIS.com xenografts.
Tait LR, Pauley RJ, Santner SJ, Heppner GH, Heng HH, Rak JW, Miller FR
International journal of cancer 2007 May 15;120(10):2127-34
International journal of cancer 2007 May 15;120(10):2127-34
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Marchal-Sommé J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Haringman JJ, Smeets TJ, Reinders-Blankert P, Tak PP
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF.
Misao Y, Takemura G, Arai M, Ohno T, Onogi H, Takahashi T, Minatoguchi S, Fujiwara T, Fujiwara H
Cardiovascular research 2006 Aug 1;71(3):455-65
Cardiovascular research 2006 Aug 1;71(3):455-65
Human melanoma metastases express functional CXCR4.
Scala S, Giuliano P, Ascierto PA, Ieranò C, Franco R, Napolitano M, Ottaiano A, Lombardi ML, Luongo M, Simeone E, Castiglia D, Mauro F, De Michele I, Calemma R, Botti G, Caracò C, Nicoletti G, Satriano RA, Castello G
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Apr 15;12(8):2427-33
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Apr 15;12(8):2427-33
Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
Meller S, Winterberg F, Gilliet M, Müller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bünemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
Arthritis and rheumatism 2005 May;52(5):1504-16
Arthritis and rheumatism 2005 May;52(5):1504-16
Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2005 Jan;23(1):203-9
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2005 Jan;23(1):203-9
Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells.
Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F
Stem cells (Dayton, Ohio) 2004;22(6):1015-29
Stem cells (Dayton, Ohio) 2004;22(6):1015-29
CXCR4 antagonist inhibits stromal cell-derived factor 1-induced migration and invasion of human pancreatic cancer.
Mori T, Doi R, Koizumi M, Toyoda E, Ito D, Kami K, Masui T, Fujimoto K, Tamamura H, Hiramatsu K, Fujii N, Imamura M
Molecular cancer therapeutics 2004 Jan;3(1):29-37
Molecular cancer therapeutics 2004 Jan;3(1):29-37
CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
Cardones AR, Murakami T, Hwang ST
Cancer research 2003 Oct 15;63(20):6751-7
Cancer research 2003 Oct 15;63(20):6751-7
CXCR4 function requires membrane cholesterol: implications for HIV infection.
Nguyen DH, Taub D
Journal of immunology (Baltimore, Md. : 1950) 2002 Apr 15;168(8):4121-6
Journal of immunology (Baltimore, Md. : 1950) 2002 Apr 15;168(8):4121-6
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Campbell JJ, Qin S, Unutmaz D, Soler D, Murphy KE, Hodge MR, Wu L, Butcher EC
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR4 in Human Spleen. CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR4 in Human Lymph Node. CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND50 is typically 2.5-12 µg/mL.
