PA1237
antibody from Boster Biological Technology
Targeting: CXCR4
CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R
Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PA1237 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CXCR4 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CXCR 4/CXCR4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CXCR 4/CXCR4 information: Molecular Weight: 39746 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC); Tissue Specificity: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references C-X-C chemokine receptor type 4 promotes tubular cell senescence and renal fibrosis through β-catenin-inhibited fatty acid oxidation.
Collagen content and C-X-C motif chemokine ligand 12 expression in neoplastic breast stroma.
RNF113A targeted by miR-197 promotes proliferation and inhibits autophagy via CXCR4/CXCL12/AKT/ERK/Beclin1 axis in cervical cancer.
MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells.
The CD133(+)CXCR4(+) Colorectal Tumor Cells Promote Colorectal Cancer Progression by PI3K/AKT Signaling.
Preferential siRNA delivery to injured kidneys for combination treatment of acute kidney injury.
CXCR4 protects bone marrow-derived endothelial progenitor cells against hypoxia through the PI3K/Akt signaling pathway.
Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells.
CXCL12 induces migration of oligodendrocyte precursor cells through the CXCR4‑activated MEK/ERK and PI3K/AKT pathways.
Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration.
Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.
CXCR4 expression in bladder transitional cell carcinoma and its relationship with clinicopathological features.
Silencing of CXCR4 inhibits tumor cell proliferation and neural invasion in human hilar cholangiocarcinoma.
Anticancer effects of chemokine receptor 4(CXCR4) gene silenced by CXCR4-siRNA in nude mice model of ovarian cancer.
Distinct roles of different fragments of PDCD4 in regulating the metastatic behavior of B16 melanoma cells.
Overexpression of CXCR4 in tracheal epithelial cells promotes their proliferation and migration to a stromal cell-derived factor-1 gradient.
CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma.
Relationships between levels of CXCR4 and VEGF and blood-borne metastasis and survival in patients with osteosarcoma.
Upregulation of CXCR4 favoring neural-like cells migration via AKT activation.
Effects of stromal-derived factor 1 preconditioning on apoptosis of rat bone mesenchymal stem cells.
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer.
SDF-1alpha/CXCR4-mediated migration of systemically transplanted bone marrow stromal cells towards ischemic brain lesion in a rat model.
Wu Q, Chen Q, Xu D, Wang X, Ye H, Li X, Xiong Y, Li J, Zhou S, Miao J, Shen W, Liu Y, Niu H, Tang Y, Zhou L
Journal of cellular and molecular medicine 2024 Feb;28(3):e18075
Journal of cellular and molecular medicine 2024 Feb;28(3):e18075
Collagen content and C-X-C motif chemokine ligand 12 expression in neoplastic breast stroma.
Ferreira CJDS, Caires IQS, Costa Neto WJBD, Almeida SMV
Revista da Associacao Medica Brasileira (1992) 2023;69(9):e20221210
Revista da Associacao Medica Brasileira (1992) 2023;69(9):e20221210
RNF113A targeted by miR-197 promotes proliferation and inhibits autophagy via CXCR4/CXCL12/AKT/ERK/Beclin1 axis in cervical cancer.
Zhang Q, Song J, Cao L, Sun M, Xu T, Yang S, Li S, Wang H, Fu X
Experimental cell research 2023 Jul 1;428(1):113632
Experimental cell research 2023 Jul 1;428(1):113632
MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells.
Zhang Y, Cao L, Du R, Tian F, Li X, Yuan Y, Wang C
PloS one 2022;17(9):e0272499
PloS one 2022;17(9):e0272499
The CD133(+)CXCR4(+) Colorectal Tumor Cells Promote Colorectal Cancer Progression by PI3K/AKT Signaling.
Guan S, Yang R, Wu S, Xu K, Yang C
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2022 May;42(5):195-202
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2022 May;42(5):195-202
Preferential siRNA delivery to injured kidneys for combination treatment of acute kidney injury.
Tang W, Chen Y, Jang HS, Hang Y, Jogdeo CM, Li J, Ding L, Zhang C, Yu A, Yu F, Foster KW, Padanilam BJ, Oupický D
Journal of controlled release : official journal of the Controlled Release Society 2022 Jan;341:300-313
Journal of controlled release : official journal of the Controlled Release Society 2022 Jan;341:300-313
CXCR4 protects bone marrow-derived endothelial progenitor cells against hypoxia through the PI3K/Akt signaling pathway.
Liu ZY, Yang QX, Cao Y, Pan HW, Rong JJ, Ling J, Tang Y, He J, Wang CL, Peng X, Zou QC, Zhang L, Zheng J, Wang J, Zhang Y, Peng JQ, Xiong LB, Liu F, Ying ZH, Zheng ZF, Zhang BL
Experimental and therapeutic medicine 2021 Nov;22(5):1200
Experimental and therapeutic medicine 2021 Nov;22(5):1200
Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells.
Ou X, Zhang GT, Tian PK, Chen JS, Lin ZW, Xie Y, Wang AH, Liu XP, Liu JK
Experimental and therapeutic medicine 2019 Aug;18(2):1091-1098
Experimental and therapeutic medicine 2019 Aug;18(2):1091-1098
CXCL12 induces migration of oligodendrocyte precursor cells through the CXCR4‑activated MEK/ERK and PI3K/AKT pathways.
Tian Y, Yin H, Deng X, Tang B, Ren X, Jiang T
Molecular medicine reports 2018 Nov;18(5):4374-4380
Molecular medicine reports 2018 Nov;18(5):4374-4380
Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration.
Hu Q, Liu M, Chen G, Xu Z, Lv Y
ACS applied materials & interfaces 2018 Aug 22;10(33):27669-27680
ACS applied materials & interfaces 2018 Aug 22;10(33):27669-27680
Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.
Yu Z, Zhao S, Ren L, Wang L, Chen Z, Hoffman RM, Zhou J
Oncotarget 2017 Sep 8;8(38):63461-63483
Oncotarget 2017 Sep 8;8(38):63461-63483
CXCR4 expression in bladder transitional cell carcinoma and its relationship with clinicopathological features.
Li Y, Chen M, Yuan J, Zhou X, He W, Zu X, Qi L
Urologia internationalis 2014;92(2):157-63
Urologia internationalis 2014;92(2):157-63
Silencing of CXCR4 inhibits tumor cell proliferation and neural invasion in human hilar cholangiocarcinoma.
Tan XY, Chang S, Liu W, Tang HH
Gut and liver 2014 Mar;8(2):196-204
Gut and liver 2014 Mar;8(2):196-204
Anticancer effects of chemokine receptor 4(CXCR4) gene silenced by CXCR4-siRNA in nude mice model of ovarian cancer.
Liu W, Wang Y, Wang H, Wang A
Cell biochemistry and biophysics 2014 Dec;70(3):1893-900
Cell biochemistry and biophysics 2014 Dec;70(3):1893-900
Distinct roles of different fragments of PDCD4 in regulating the metastatic behavior of B16 melanoma cells.
Wang D, Guo S, Han SY, Xu N, Guo JY, Sun Q
International journal of oncology 2013 May;42(5):1725-33
International journal of oncology 2013 May;42(5):1725-33
Overexpression of CXCR4 in tracheal epithelial cells promotes their proliferation and migration to a stromal cell-derived factor-1 gradient.
Liu J, Yang X, Shi W
Experimental biology and medicine (Maywood, N.J.) 2013 Feb;238(2):144-50
Experimental biology and medicine (Maywood, N.J.) 2013 Feb;238(2):144-50
CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma.
Zhao BC, Wang ZJ, Mao WZ, Ma HC, Han JG, Zhao B, Xu HM
World journal of gastroenterology 2011 May 21;17(19):2389-96
World journal of gastroenterology 2011 May 21;17(19):2389-96
Relationships between levels of CXCR4 and VEGF and blood-borne metastasis and survival in patients with osteosarcoma.
Lin F, Zheng SE, Shen Z, Tang LN, Chen P, Sun YJ, Zhao H, Yao Y
Medical oncology (Northwood, London, England) 2011 Jun;28(2):649-53
Medical oncology (Northwood, London, England) 2011 Jun;28(2):649-53
Upregulation of CXCR4 favoring neural-like cells migration via AKT activation.
Li S, Deng L, Gong L, Bian H, Dai Y, Wang Y
Neuroscience research 2010 Aug;67(4):293-9
Neuroscience research 2010 Aug;67(4):293-9
Effects of stromal-derived factor 1 preconditioning on apoptosis of rat bone mesenchymal stem cells.
Chen J, Du X, Zhang K
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Aug;29(4):423-6
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Aug;29(4):423-6
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer.
Zhang S, Qi L, Li M, Zhang D, Xu S, Wang N, Sun B
Journal of experimental & clinical cancer research : CR 2008 Nov 4;27(1):62
Journal of experimental & clinical cancer research : CR 2008 Nov 4;27(1):62
SDF-1alpha/CXCR4-mediated migration of systemically transplanted bone marrow stromal cells towards ischemic brain lesion in a rat model.
Wang Y, Deng Y, Zhou GQ
Brain research 2008 Feb 21;1195:104-12
Brain research 2008 Feb 21;1195:104-12
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237). CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image