Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP2-27500 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal RRAD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Reported variants represent identical protein: NP_004156.1, NP_001122322.1
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment.
Lee I, Yeom SY, Lee SJ, Kang WK, Park C
Cancer research 2010 Jun 1;70(11):4357-65
Cancer research 2010 Jun 1;70(11):4357-65
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: RRAD Antibody [NBP2-27500] - Panel A: Staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) with antibody at 0.5 ug/mL, and compared with an HA-specific antibody. Panel B: Staining of WT and KO lysates of Mouse Heart (100 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.