Explore
Validate
LearnPA5-34505
antibody from Invitrogen Antibodies
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-34505 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCP-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat spleen tissue lysate. PA5-34505 can be used with blocking peptide PEP-1548.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Hdac8 Inhibitor Alleviates Transverse Aortic Constriction-Induced Heart Failure in Mice by Downregulating Ace1.
The hepatocyte growth factor/c-met pathway is a key determinant of the fibrotic kidney local microenvironment.
Use of magnetic capture to identify elevated levels of CCL2 following intra-articular injection of monoiodoacetate in rats.
Pre-eclamptic Fetal Programming Alters Neuroinflammatory and Cardiovascular Consequences of Endotoxemia in Sex-Specific Manners.
Zhao T, Kee HJ, Kee SJ, Jeong MH
Oxidative medicine and cellular longevity 2022;2022:6227330
Oxidative medicine and cellular longevity 2022;2022:6227330
The hepatocyte growth factor/c-met pathway is a key determinant of the fibrotic kidney local microenvironment.
Fu H, Gui Y, Liu S, Wang Y, Bastacky SI, Qiao Y, Zhang R, Bonin C, Hargis G, Yu Y, Kreutzer DL, Biswas PS, Zhou Y, Wang Y, Tian XJ, Liu Y, Zhou D
iScience 2021 Oct 22;24(10):103112
iScience 2021 Oct 22;24(10):103112
Use of magnetic capture to identify elevated levels of CCL2 following intra-articular injection of monoiodoacetate in rats.
Yarmola EG, Shah YY, Lakes EH, Pacheco YC, Xie DF, Dobson J, Allen KD
Connective tissue research 2020 Sep;61(5):485-497
Connective tissue research 2020 Sep;61(5):485-497
Pre-eclamptic Fetal Programming Alters Neuroinflammatory and Cardiovascular Consequences of Endotoxemia in Sex-Specific Manners.
Abuiessa SA, Wedn AM, El-Gowilly SM, Helmy MM, El-Mas MM
The Journal of pharmacology and experimental therapeutics 2020 May;373(2):325-336
The Journal of pharmacology and experimental therapeutics 2020 May;373(2):325-336
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat spleen tissue lysate using a CCL2 polyclonal antibody (Product # PA5-34505) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CCL2 in K562 cell lysate with MCP-1 Polyclonal Antibody (Product # PA5-34505) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human spleen tissue using a CCL2 polyclonal antibody (Product # PA5-34505) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of CCL2 in human spleen tissue with MCP-1 Polyclonal Antibody (Product # PA5-34505) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of CCL2 in human spleen tissue with MCP-1 Polyclonal Antibody (Product # PA5-34505) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Macrophage-specific deletion of c-met enhances fibrosis but has little effect on extracellular matrix (ECM) synthesis (A) Schematic diagram showing the generation of macrophage-specific c-met deletion mice. (B and C) qPCR analyses of FN , alpha-SMA , type I collagen , and type III collagen mRNA in Lyz-met+/+ and Lyz-met-/- mice kidneys after IRI at 10 days and UUO at 7 (1 week [w]) and 14 days (2 w). n = 4-5. (D-G) Western blot analyses of FN, alpha-SMA, and TNC protein expression in Lyz-met+/+ and Lyz-met-/- mice kidneys after IRI (D) and UUO (F). Quantification of protein expression for IRI (E) and UUO (G). Dots indicate individual animals within each group. n = 3-5. (H and I) Representative micrographs for Sirius red, Masson's trichrome, and FN staining in Lyz-met+/+ and Lyz-met-/- mice kidneys after IRI and UUO, respectively. Scale bar, 50 mum. (J) Bar graph of collagen/non-collagen ratios in Lyz-met+/+ and Lyz-met-/- mice kidneys after IRI and UUO, respectively. n = 3-5. (K) qPCR analyses of MCP-1 mRNA in Lyz-met+/+ and Lyz-met-/- kidneys after IRI. n = 4. (L) qPCR analysis of MCP-1 mRNA in Lyz-met+/+ and Lyz-met-/- mice kidneys after UUO. n = 4. (M and N) Western blot analyses (M) and quantitative data (N) of MCP-1 protein expression in Lyz-met +/+ and Lyz-met -/- mice kidneys after IRI. Dots indicate individual animals within each group. n = 3. (O and P) Western blot analyses (O) and quantitative data (P) demonstrating MCP-1 protein expression in Lyz-met+/+ an
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 PCI34051 downregulates the expression of Hdac8 and inflammation-related genes in transverse aortic constriction (TAC) mice. (a-j) Cardiac and pulmonary mRNA levels of Hdac8 , Il1b , Ccl2 , Rela , and Nfkbia in the sham+vehicle, sham+PCI34051 (10 mg/kg bodyweight/day), TAC+vehicle, and TAC+PCI34051 (3, 10, or 30 mg/kg bodyweight/day) groups were determined using quantitative real-time polymerase chain reaction. (k) Cardiac expression levels of Hdac8, Ccl2, Rela, and Nfkbia; representative western blot images are shown. Actb was used as a loading control. (l-o) Quantification of Hdac8, Ccl2, Rela, and Nfkbia levels ( n = 5-6 per group). (p) Pulmonary expression levels of Hdac8, Ccl2, Rela, and Nfkbia; representative western blot images. Actb was used as a loading control. (q-t) Quantification of Hdac8, Ccl2, Rela, and Nfkbia levels ( n = 5-6 per group).
