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LearnARG54724
antibody from Arigo
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG54724 - Provider product page
- Provider
- Arigo
- Product name
- anti-MCP-1 / CCL2 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 62-89 (C-terminus) of Human CCL2 protein (NP_002973.1).
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of Hela cell line lysates stained with ARG54724 anti-MCP-1 / CCL2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Hela cells stained with ARG54724 anti-MCP-1 / CCL2 antibody at 0.025 mg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Hela cell stained with ARG54724 anti-MCP-1 / CCL2 antibody followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: human brain tissue stained with ARG54724 anti-MCP-1 / CCL2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG54724 anti-MCP-1 / CCL2 antibody (bottom histogram) or without primary antibody control (top histogram).
