Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-117680 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RANBP10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2.11 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RANBP10 using RANBP10 Polyclonal Antibody (Product # PA5-117680) at 2.1 µg/mL. Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 68, 59, 65 kDa. Observed band size: 68 kDa
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RANBP10 using RANBP10 Polyclonal Antibody (Product # PA5-117680) at 2.1 µg/mL. Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 68, 59, 65 kDa. Observed band size: 68 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of RANBP10 in paraffin-embedded human skeletal muscle tissue using RANBP10 Polyclonal Antibody (Product # PA5-117680) diluted at 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating RANBP10 in Rat Brain tissue. Lane 1: Rabbit control IgG Rat Brain tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: RANBP10 Polyclonal Antibody (Product # PA5-117680) (6 µg) + Rat Brain tissue (1mg). Lane 3: Rat Brain tissue (20 µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating RANBP10 in Rat Brain tissue. Lane 1: Rabbit control IgG Rat Brain tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: RANBP10 Polyclonal Antibody (Product # PA5-117680) (6 µg) + Rat Brain tissue (1mg). Lane 3: Rat Brain tissue (20 µg).