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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- NBP2-27329 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SODD/BAG4 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Expression of Bcl-2 and the antiapoptotic BAG family proteins in ovarian cancer.
Aust S, Pils S, Polterauer S, Horvat R, Cacsire Castillo-Tong D, Pils D, Dudek G, Schmid B, Speiser P, Reinthaller A, Grimm C
Applied immunohistochemistry & molecular morphology : AIMM 2013 Dec;21(6):518-24
Applied immunohistochemistry & molecular morphology : AIMM 2013 Dec;21(6):518-24
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: SODD/BAG4 Antibody [NBP2-27329] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using SODD/BAG4 antibody at 1:5000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Connective tissue staining was observed. Staining was performed by Histowiz.
