Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX115986 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115986, RRID:AB_11175611
- Product name
- CREST antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE GTX115986 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREST antibody [C1C3] detects CREST protein by western blot analysis. U87-MG whole cell extracts and nuclear extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] (GTX115986) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREST antibody [C1C3] detects CREST protein by western blot analysis. Rat tissue extracts (50 £gg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] (GTX115986) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREST antibody [C1C3] detects CREST protein by western blot analysis. Mouse tissue extracts (50 £gg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] (GTX115986) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREST antibody [C1C3] detects CREST protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CREST protein stained by CREST antibody [C1C3] (GTX115986) diluted at 1:500.Blue: Hoechst 33342 staining.