Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CREST Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLa nucleus, mouse brain.
- Concentration
- 0.73 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CREST Polyclonal Antibody (Product # PA5-30500). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. CREST Polyclonal Antibody (Product # PA5-30500) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CREST Polyclonal Antibody (Product # PA5-30500). Sample (20 µg ). Lane A: Hela whole cell lysate. Lane B: HeLa nucleus 10% SDS PAGE. CREST Polyclonal Antibody (Product # PA5-30500) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CREST in paraformaldehyde-fixed A431 cells using a CREST polyclonal antibody (Product # PA5-30500) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using CREST (Product # PA5-30500) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.