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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
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- Product number
- GTX83771 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83771, RRID:AB_10729289
- Product name
- PTCH1 antibody [5c7]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex.
Activities of the Cytoplasmic Domains of Patched-1 Modulate but Are Not Essential for the Regulation of Canonical Hedgehog Signaling.
Qi X, Schmiege P, Coutavas E, Li X
Science (New York, N.Y.) 2018 Oct 5;362(6410)
Science (New York, N.Y.) 2018 Oct 5;362(6410)
Activities of the Cytoplasmic Domains of Patched-1 Modulate but Are Not Essential for the Regulation of Canonical Hedgehog Signaling.
Fleet A, Lee JP, Tamachi A, Javeed I, Hamel PA
The Journal of biological chemistry 2016 Aug 19;291(34):17557-68
The Journal of biological chemistry 2016 Aug 19;291(34):17557-68
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Supportive validation
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-PTCH1 mouse monoclonal antibody (GTX83771) immunofluorescent staining of COS7 cells transiently transfected with PTCH1
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PTCH1 mouse monoclonal antibody. (GTX83771)
