PA1-46222

antibody from Invitrogen Antibodies

Targeting: PTCH1 BCNS, NBCCS, PTCH

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-46222

Provider

Invitrogen Antibodies

Product name

PTCH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: antigen standard for PTCH1 (transient overexpression lysate).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway.

Sun Q, Zeng J, Liu Y, Chen J, Zeng QC, Chen YQ, Tu LL, Chen P, Yang F, Zhang M
Aging 2020 Jun 16;12(12):11446-11465

---

The Expression of Shh, Ptch1, and Gli1 in the Developing Caudal Spinal Cord of Fetal Rats With Anorectal Malformations.

Yang Z, Gao L, Jia H, Bai Y, Wang W
The Journal of surgical research 2019 Jan;233:173-182

---

Sonic hedgehog expression is disrupted following in ovo ethanol exposure during early chick eye development.

Brennan D, Giles S
Reproductive toxicology (Elmsford, N.Y.) 2013 Nov;41:49-56

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTCH in fetal brain lysate. Samples were incubated in PTCH polyclonal antibody (Product # PA1-46222) using a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 miR-9 and miR-29a regulate ISL1-mediated SHH signaling pathway. ( A ) The relative expression of ISL1 after lentiviral infection determined by RT-qPCR. ( B ) mRNA expression of SHH, GLI1, and PTCH in serum of rats after lentiviral infection detected by RT-qPCR. ( C ) The protein bands and expression of SHH, GLI1, and PTCH in serum of rats after lentiviral infection normalized to GAPDH detected by Western blot analysis; * p < 0.05 vs. the LV-NC group; # p < 0.05 vs. the LV-ISL1 group. ( D ) The effect of miR-9 and miR-29a on mRNA expression of SHH, GLI1, and PTCH in serum of rats detected by RT-qPCR. ( E ) The effect of miR-9 and miR-29a on protein bands and expression of SHH, GLI1, and PTCH in serum of rats normalized to GAPDH detected by Western blot analysis; * p < 0.05 vs. the normal group; # p < 0.05 vs. the DM group; @ p < 0.05 vs. the LV-ISL1 + LV-miR-9 group; & p < 0.05 vs. the LV-ISL1 + LV-miR-29a group. Data were measurement data and expressed as mean +- standard deviation; t test was performed for pairwise comparison; one-way analysis of variance was performed for multiple-group comparison; n = 8; the experiment was repeated three times independently.