AF3468

antibody from R&D Systems

Targeting: PSIP1 DFS70, LEDGF, p52, p75, PSIP2

Antibody data

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Validation data

Product number: AF3468 - Provider product page
Provider: R&D Systems
Product name: Human LEDGF Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human LEDGF in direct ELISAs and Western blots.
Reactivity: Human
Host: Goat
Conjugate: Unconjugated
Antigen sequence: O75475
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

PSIP1 protein structure - AF3468 shown in red.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human LEDGF by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human CD4⁺ T cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human LEDGF by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LEDGF p75 at approximately 99 kDa (as indicated) using 5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Western Blot Shows Human LEDGF Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LEDGF knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 49 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.