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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP12263PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12263PU-N, RRID:AB_1752307
- Product name
- anti ACSL4
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 244~274 amino acids from the center region of human FACL4.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-FACL4 (Center) Pab (Cat.#AP12263PU-N) to detect FACL4 in RKO cell lysate (human colon cancer) treated with 10% FCS for 24 hrs. Data is kindly provided by Drs. M.G. Sciulli and P. Patrignani from the University of Chieti (Italy).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ACSL4 (FACL4) Antibody (Center) Cat.-No AP12263PU-N with Hela cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
