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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX100260 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100260, RRID:AB_1240790
- Product name
- FACL4 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death.
The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells.
Mitofusin 2 ablation increases endoplasmic reticulum-mitochondria coupling.
Garcia-Bermudez J, Baudrier L, Bayraktar EC, Shen Y, La K, Guarecuco R, Yucel B, Fiore D, Tavora B, Freinkman E, Chan SH, Lewis C, Min W, Inghirami G, Sabatini DM, Birsoy K
Nature 2019 Mar;567(7746):118-122
Nature 2019 Mar;567(7746):118-122
The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells.
Radif Y, Ndiaye H, Kalantzi V, Jacobs R, Hall A, Minogue S, Waugh MG
Molecular and cellular biochemistry 2018 Nov;448(1-2):275-286
Molecular and cellular biochemistry 2018 Nov;448(1-2):275-286
Mitofusin 2 ablation increases endoplasmic reticulum-mitochondria coupling.
Filadi R, Greotti E, Turacchio G, Luini A, Pozzan T, Pizzo P
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 28;112(17):E2174-81
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 28;112(17):E2174-81
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Supportive validation
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa B: NT2D1 7.5% SDS PAGE GTX100260 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX100260 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (GTX100260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FACL4(GTX100260) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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- Main image
- Experimental details
- FACL4 antibody [C3], C-term detects FACL4 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FACL4 protein stained by FACL4 antibody [C3], C-term (GTX100260) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ?g of FACL4 antibody [C3], C-term (GTX100260).Western blot analysis was performed using FACL4 antibody [C3], C-term (GTX100260).EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using FACL4(GTX100260) antibody at 1:500 dilution.
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- GeneTex (provider)
- Main image
- Experimental details
- FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on mouse lymph node by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. FACL4 antibody [C3], C-term (GTX100260) dilution: 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. FACL4 antibody [C3], C-term (GTX100260) dilution: 1:500.
