Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX114399 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114399, RRID:AB_10619561
- Product name
- FACL4 antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE FACL4 antibody GTX114399 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (GTX114399) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACL4 antibody [N1N3] detects FACL4 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FACL4 protein stained by FACL4 antibody [N1N3] (GTX114399) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACL4 antibody [N1N3] detects FACL4 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 antibody [N1N3] (GTX114399) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACL4 antibody [N1N3] detects FACL4 protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. FACL4 antibody [N1N3] (GTX114399) diluted at 1:500.