Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 605302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#605302, RRID:AB_2174072
- Product name
- Purified anti-PYK2
- Antibody type
- Polyclonal
- Antigen
- Recombinant (partial), N-terminal
- Description
- PYK2, a 116 kD proline-rich non-receptor tyrosine kinase also known as FAK2 or PTK2B, is ubiquitously expressed in the cytoplasm. PYK2 is activated in response to integrin ligation, CD3 or TCR ligation, growth factors, cytokines, chemokines, and cellular stress. This kinase has been shown to regulate ion channels, vesicular transport, participate in T- and B-cell receptor signaling, and play a role in apoptosis. PYK2 is activated through phosphorylation following increased intracellular calcium and can be dephosphorylated by calcitonin, and protein tyrosine phosphatase (non-receptor) type 12 to decrease kinase activity. PYK2 has been shown to interact with Grb2, SHP1, integrin β3, Fyn, c-Src, talin, paxillin, Vav-1, Lyn, and HIC5. The Poly6053 antibody recognizes the N-terminal region of human, mouse, and rat PYK2 and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Role of Pyk2 in cardiac arrhythmogenesis.
Lang D, Glukhov AV, Efimova T, Efimov IR
American journal of physiology. Heart and circulatory physiology 2011 Sep;301(3):H975-83
American journal of physiology. Heart and circulatory physiology 2011 Sep;301(3):H975-83
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PYK2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated