11510-1-AP

antibody from Invitrogen Antibodies

Targeting: YME1L1 YME1L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11510-1-AP

Provider

Invitrogen Antibodies

Product name

YME1L1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GSEFDEMFV GVGASRIRNL FREAKANAPC VIFIDELDSV GGKRIESPMH PYSRQTINQL LAEMDGFKPN EGVIIIGATN FPEALDNALI RPGRFDMQVT VPRPDVKGRT EILKWYLNKI KFDQSVDPEI IARGTVGFSG AELENLVNQA ALKAAVDGKE MVTMKELEFS KDKILMGPER RSVEIDNKNK TITAYHESGH AIIAYYTKDA MPINKATIMP RGPTLGHVSL LPENDRWNET RAQLLAQMDV SMGGRVAEEL IFGTDHITTG ASSDFDNATK IAKRMVTKFG MSEKLGVMTY SDTGKLSPET QSAIEQEIRI LLRDSYERAK HILKTHAKEH KNLAEALLTY ETLDAKEIQI VLEGKKLEVR (348-716 aa encoded by BC023507)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 11510-1-AP (YME1L1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11510-1-AP (YME1L1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11510-1-AP (YME1L1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11510-1-AP ( YME1L1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11510-1-AP ( YME1L1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-YME1L1 (IP:11510-1-AP, 4ug; Detection:11510-1-AP 1:500) with HeLa cells lysate 1280 ug.