Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 12295-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12295-1-AP, RRID:AB_2164779
- Product name
- Prohibitin 2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Prohibitin 2 antibody (Cat. #12295-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Sensitive electrochemical immunosensor to detect prohibitin 2, a potential blood cancer biomarker.
Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.
Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.
Nitazoxanide impairs mitophagy flux through ROS-mediated mitophagy initiation and lysosomal dysfunction in bladder cancer.
BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.
SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2.
ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.
PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.
Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA.
Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.
Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.
NAD+ repletion inhibits the endothelial-to-mesenchymal transition induced by TGF-β in endothelial cells through improving mitochondrial unfolded protein response.
Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes.
Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.
PHB2 interacts with LC3 and SQSTM1 is required for bile acids-induced mitophagy in cholestatic liver.
Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.
Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
Alterations of mitochondrial protein assembly and jasmonic acid biosynthesis pathway in Honglian (HL)-type cytoplasmic male sterility rice.
Proteomic analysis identifies in vivo candidate matrix metalloproteinase-9 substrates in the left ventricle post-myocardial infarction.
Yun YR, Lee SY, Seo B, Kim H, Shin MG, Yang S
Talanta 2022 Feb 1;238(Pt 2):123053
Talanta 2022 Feb 1;238(Pt 2):123053
Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.
Chamberlain KA, Huang N, Xie Y, LiCausi F, Li S, Li Y, Sheng ZH
Neuron 2021 Nov 3;109(21):3456-3472.e8
Neuron 2021 Nov 3;109(21):3456-3472.e8
Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.
Wang W, Zhai D, Bai Y, Xue K, Deng L, Ma L, Du T, Ye Z, Qu D, Xiang A, Chen G, Zhao Y, Wang L, Lu Z
Cell death discovery 2021 Mar 23;7(1):58
Cell death discovery 2021 Mar 23;7(1):58
Nitazoxanide impairs mitophagy flux through ROS-mediated mitophagy initiation and lysosomal dysfunction in bladder cancer.
Sun H, Ou T, Hu J, Yang Z, Lei Q, Li Y, Wang G, Li Y, Wu K, Wang S, Wu S
Biochemical pharmacology 2021 Aug;190:114588
Biochemical pharmacology 2021 Aug;190:114588
BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.
Wu X, Zheng Y, Liu M, Li Y, Ma S, Tang W, Yan W, Cao M, Zheng W, Jiang L, Wu J, Han F, Qin Z, Fang L, Hu W, Chen Z, Zhang X
Autophagy 2021 Aug;17(8):1934-1946
Autophagy 2021 Aug;17(8):1934-1946
SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2.
von Wenserski L, Schultheiß C, Bolz S, Schliffke S, Simnica D, Willscher E, Gerull H, Wolters-Eisfeld G, Riecken K, Fehse B, Altfeld M, Nollau P, Binder M
Leukemia 2021 Apr;35(4):1073-1086
Leukemia 2021 Apr;35(4):1073-1086
ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.
Shu L, Hu C, Xu M, Yu J, He H, Lin J, Sha H, Lu B, Engelender S, Guan M, Song Z
The EMBO journal 2021 Apr 15;40(8):e106283
The EMBO journal 2021 Apr 15;40(8):e106283
PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.
Yan C, Gong L, Chen L, Xu M, Abou-Hamdan H, Tang M, Désaubry L, Song Z
Autophagy 2020 Mar;16(3):419-434
Autophagy 2020 Mar;16(3):419-434
Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA.
Hao Q, Wang P, Dutta P, Chung S, Li Q, Wang K, Li J, Cao W, Deng W, Geng Q, Schrode K, Shaheen M, Wu K, Zhu D, Chen QH, Chen G, Elshimali Y, Vadgama J, Wu Y
Cell death & disease 2020 Dec 11;11(12):1052
Cell death & disease 2020 Dec 11;11(12):1052
Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.
Cho SG, Xiao X, Wang S, Gao H, Rafikov R, Black S, Huang S, Ding HF, Yoon Y, Kirken RA, Yin XM, Wang HG, Dong Z
Journal of the American Society of Nephrology : JASN 2019 Jul;30(7):1174-1191
Journal of the American Society of Nephrology : JASN 2019 Jul;30(7):1174-1191
Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.
Han Y, Wu P, Wang Z, Zhang Z, Sun S, Liu J, Gong S, Gao P, Iwakuma T, Molina-Vila MA, Chen BP, Zhang Y, Ji T, Mo Q, Chen P, Hu J, Wang S, Zhou J, Lu H, Gao Q
EBioMedicine 2019 Feb;40:92-105
EBioMedicine 2019 Feb;40:92-105
NAD+ repletion inhibits the endothelial-to-mesenchymal transition induced by TGF-β in endothelial cells through improving mitochondrial unfolded protein response.
Zhang M, Weng H, Zheng J
The international journal of biochemistry & cell biology 2019 Dec;117:105635
The international journal of biochemistry & cell biology 2019 Dec;117:105635
Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes.
Yahiro K, Ogura K, Terasaki Y, Satoh M, Miyagi S, Terasaki M, Yamasaki E, Moss J
Cellular microbiology 2019 Aug;21(8):e13033
Cellular microbiology 2019 Aug;21(8):e13033
Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.
Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005
Cell reports 2018 Jun 5;23(10):2989-3005
PHB2 interacts with LC3 and SQSTM1 is required for bile acids-induced mitophagy in cholestatic liver.
Xiao Y, Zhou Y, Lu Y, Zhou K, Cai W
Cell death & disease 2018 Feb 7;9(2):160
Cell death & disease 2018 Feb 7;9(2):160
Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
Li H, Ruan Y, Zhang K, Jian F, Hu C, Miao L, Gong L, Sun L, Zhang X, Chen S, Chen H, Liu D, Song Z
Cell death and differentiation 2016 Mar;23(3):380-92
Cell death and differentiation 2016 Mar;23(3):380-92
Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.
Otera H, Miyata N, Kuge O, Mihara K
The Journal of cell biology 2016 Feb 29;212(5):531-44
The Journal of cell biology 2016 Feb 29;212(5):531-44
Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
Poitelon Y, Bogni S, Matafora V, Della-Flora Nunes G, Hurley E, Ghidinelli M, Katzenellenbogen BS, Taveggia C, Silvestri N, Bachi A, Sannino A, Wrabetz L, Feltri ML
Nature communications 2015 Sep 18;6:8303
Nature communications 2015 Sep 18;6:8303
Alterations of mitochondrial protein assembly and jasmonic acid biosynthesis pathway in Honglian (HL)-type cytoplasmic male sterility rice.
Liu G, Tian H, Huang YQ, Hu J, Ji YX, Li SQ, Feng YQ, Guo L, Zhu YG
The Journal of biological chemistry 2012 Nov 16;287(47):40051-60
The Journal of biological chemistry 2012 Nov 16;287(47):40051-60
Proteomic analysis identifies in vivo candidate matrix metalloproteinase-9 substrates in the left ventricle post-myocardial infarction.
Zamilpa R, Lopez EF, Chiao YA, Dai Q, Escobar GP, Hakala K, Weintraub ST, Lindsey ML
Proteomics 2010 Jun;10(11):2214-23
Proteomics 2010 Jun;10(11):2214-23
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12295-1-AP(PHB2 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PHB2 antibody 12295-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-PHB2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 12295-1-AP(PHB2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 12295-1-AP(PHB2 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue