10587-1-AP

antibody from Invitrogen Antibodies

Targeting: RBM15 OTT, OTT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10587-1-AP

Provider

Invitrogen Antibodies

Product name

RBM15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SSSRDRYNS DNDRSSRLLL ERPSPIRDRR GSLEKSQGDK RDRKNSASAE RDRKHRTTAP TEGKSPLKKE DRSDGSAPST STASSKLKSP SQKQDGGTAP VASASPKLCL AWQGMLLLKN SNFPSNMHLL QGDLQVASSL LVEGSTGGKV AQLKITQRLR LDQPKLDEVT RRIKVAGPNG YAILLAVPGS SDSRSSSSSA ASDTATSTQR PLRNLVSYLK QKQAAGVISL PVGGNKDKEN TGVLHAFPPC EFSQQFLDSP AKALAKSEED YLVMIIVRGF GFQIGVRYEN KKRENLALTL L (677-977 aa encoded by BC006397)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.32 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of RBM15 antibody (10587-1-AP, 1:3000) with si-control and si-RBM15 transfected HEK293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RBM15 antibody 10587-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10587-1-AP ( RBM15 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RBM15 (IP:10587-1-AP, 4ug; Detection:10587-1-AP 1:500) with HEK-293 cells lysate 2600 ug.